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Question
A method used to insert or transform cells with a plasmid is to:
a. add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall.
b. add the DNA to a heated suspension of cells at 42°C.
c. treat the bacteria with Ca2+, add the DNA, and briefly heat to 42°C.
d. incubate the DNA with the cells overnight at 4°C.
e. mixing plasmids with an extract of broken cells.
Answer
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Related questions
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b. Hsp90; un-; chaperonin
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e. Hsp60; partially; Hsp40
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In a class I aminoacyl-tRNA synthetase, which of the following reacts with the acyl phosphate formed during the reaction of ATP with the amino acid?a. 5'-hydroxyl of the nucleotide on the 5' end of the tRNA moleculeb. 3'-hydroxyl of the nucleotide on the 5' end of the tRNA moleculec. 2'-hydroxyl of the nucleotide on the 5' end of the tRNA moleculed. 3'-hydroxyl of the nucleotide on the 3' end of the tRNA moleculee. 2'-hydroxyl of the nucleotide on the 3' end of the tRNA molecule
Q:
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Q:
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Q:
Termination of translation in prokaryotic cells requires:
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b. interaction of release factors with the termination codon.
c. ternary interaction of the release factor and the termination tRNA with the termination codon.
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Q:
Which of the following does not reqire GTP hydrolysis?
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Q:
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Q:
Mitochondrial and chloroplastic ribosomes resemble ____ ribosomes.
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c. higher eukaryotic
d. no other
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Q:
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b. asp; ala; polycationic
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Q:
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Q:
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Q:
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Q:
The linkage between the amino acid and the tRNA in an amino-acyl tRNA complex is:
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Q:
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c. allose
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Q:
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Q:
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Q:
The base-pair edges in the ____ groove act as a(n) ____ identifiable through ____ with specific proteins.
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Q:
The eukaryotic transcription factor that exhibits a sequence specificity for the TATA box is:
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Q:
All are correct about enhancer sequences in eukaryotic cells EXCEPT:
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Q:
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Q:
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Q:
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Q:
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b. a base analog such as 5-bromouracil
c. a chemical mutagen such as nitrous acid
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Q:
Which of the following statements explains why the adenine analog, 2-aminopurine (2-AP), can cause mutations in DNA when it is inserted in place of a site normally occupied by A?
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e. none of the above
Q:
Misincorporation of all subsequent amino acids results from ____ mutations.
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Q:
Mutations in DNA may result from all of the following EXCEPT:
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b. physical insults on the cell, such as UV light.
c. errors in transcription.
d. chemical mutagens.
e. introduction of a base analog into DNA.
Q:
A transition mutation would be replacing A by:
a. T.
b. C.
c. U.
d. G.
e. either T or C