Question

Enumerating the viable and total cell concentration of a population of a microbial isolate can be a really laborious task for a microbiologist. Especially when a microbiologist studies the same isolate for several years, it often becomes practical to determine the relationship between optical density (OD) and cell concentration. Once this relationship (determined by a standard curve) is determined, the OD of an isolate in a broth can be routinely used to determine the population's concentration. Why must a standard curve be prepared for each isolate when using OD measurements to determine cell concentration? Also describe an experiment that would generate this type of standard curve.

Answer

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