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Question

For an enzyme-catalyzed reaction, the initial velocity was determined at two different concentrations of the substrate. Which of the following would be closest to the value of Km?
[S] (mM) Vo(mM/min)
1.0 2.0
4.0 2.8

a. 0.17 mM
b. 5.7 mM
c. 2.7 mM
d. 0.60 mM
e. 1.7 mM

Answer

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