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Question
Insulin is a polypeptide hormone that contains two short polypeptide chains linked by two interstrand disulfide bonds. The most logical order of events to perform in order to sequence this protein would be:A. The peptides are reduced with mercaptoethanol.
B. The peptides are sequenced using Edman chemistry.
C. The peptides are separated by chromatography techniques.
D. The peptides are alkylated with iodoacetamide.
a. A, D, C, B
b. C, A, D, B
c. C, B, A, D
d. A, B, C, D
e. A, C, D, B
Answer
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c. U.
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