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Question
Reaction of the peptide, ala-met-lys-ser, with phenylisothiocyanate (PITC) at pH 8.0 followed by mild acidification (first cycle of Edman method) would release:a. the labeled peptide ala-met-lys-ser-PTH.
b. PTH-ala, PTH-ser, PTH-lys and PTH-met.
c. PTH-ser and the peptide ala-met-lys.
d. PTH-ala and the peptide met-lys-ser.
e. All of the above.
Answer
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