Question

The appropriate sequence for ubiquitination of proteins to be degraded is:
A. multiple ubiquitinations may occur on a protein substrate,
B. ubiquitin-protein ligase (E3) transfers ubiquitin to free amino groups on the protein,
C. E3 selects a protein for degradation by the nature of the N-terminal amino acid,
D. ubiquitin-carrier protein (E2) picks up ubiquitin,
E. ubiquitin-activating enzyme (E1) attaches via ATP-dependent formation of thioester bond to C-termini of ubiquitin,
a. A, C, B, D, E
b. E, D, A, B, C
c. D, E, C, A, B
d. C, E, D, B, A
e. E, D, C, B, A

Answer

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