Question

You have performed the following mating experiment using Hfr and F- strains of Escherichia coli:
Hfr (thr+ leu+ gal+ strs) F- (thr- leu- gal- strr). Which of the following selective media would you use to score recombinant colonies?
A) minimal medium
B) minimal medium + streptomycin
C) minimal medium + threonine
D) minimal medium + streptomycin + threonine

Answer

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