Question

You isolate a purify a bacteriophage that can replicate in E. coli. Through chemical analyses you determine that the only nucleic acid present is RNA. You isolate the RNA and put it in a test tube with all of the necessary proteins and RNAs for translation. The RNA is translated and new infectious virions are made. What does this tell you about the bacteriophage?
A) The RNA genome is of the plus sense.
B) The RNA genome is of the plus sense and RNA replicase is present in the viral capsid.
C) The new bacteriophage is a retrovirus.
D) RNA replicase is present in the viral capsid.

Answer

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